Journal: The Journal of Neuroscience

Article Title: Ubiquitin-Specific Protease 2 in the Ventromedial Hypothalamus Modifies Blood Glucose Levels by Controlling Sympathetic Nervous Activation

doi: 10.1523/jneurosci.2504-21.2022

Figure Lengend Snippet: Figure 1. Expression of Usp2 mRNA in the mouse brain. A–G, I, J, Chromogenic (A–F, I, J) and fluorescent (G, I, J) ISH using antisense (A, C–G, I, J) or sense (B) probes for Usp2 transcripts; sagittal (A–D) and coronal (E–G, I, J) sections of adult mouse brains are shown, with highly magnified images of the hippocampus (C), cerebellar (D), and hypothalamic (E–G, I, J) regions. G, I, J, Combinatory technique of fluorescent ISH (Usp2 mRNA and Eaat1 mRNA) and immunofluorescence detection (NeuN) in VMH (G), PVN (I), and ARC and DMH (J). H, Distribution of Usp2 mRNA1 to NeuN1 or Eaat1 mRNA1 cells in the parenchymal region of the VMH. The propor- tion of Usp2 mRNA1 area colocalized with NeuN1 or Eaat1 mRNA1 were calculated. I, J, Right, Lower and higher magnification of hypothalamic nuclei. E, F, I, J, The hypothalamic nuclei are surrounded by yellow dashed lines. Blue arrowhead indicates the cerebellar granular layer (D). G, I, J, Arrows indicate Usp2 mRNA signal on NeuN1

Article Snippet: To generate Usp2 cRNA probes, a mouse Usp2 cDNA fragment (accession no. NM_198091, GenBank; corresponding with the 44 000,429- 44 005,159 bp of the mouse genome version GRCm 38.p2) was cloned into a pBluescriptII plasmid vector (Addgene).

Techniques: Expressing, Immunofluorescence